Project Report

Incidence of Asymptomatic UTI & Antibiotics Susceptibility Pattern of Isolates in Primary School Going Children of Kathmandu Valley

Dhiraj Kr. Chaudhary  , Malavika Gupta,  Prabin Bhandari,  Reena Maharjan, Siyaram Bhagat

ABSTRACT

Urinary tract infection is one of the most common bacterial infections during childhood. It is caused by microbial invasion of tissue lining the urinary tract which extends from renal cortex to urethral meatus. Asymptomatic UTI is defined as a significant bacterial count (usually10⁵organism/ml) present in the urine of a person without symptoms.

The main purpose of the study is to find out the incidence of asymptomatic UTI in primary school going children of different age groups & sex and to know the antibiotic susceptibility pattern of the isolates.

The study was carried out in Microbiology Laboratory of St. Xavier’s College, Maitighar, Kathmandu from February 6-March 21 ,2011. A total of 54 midstream urine sample of age 2-12 years children from different school of Kathmandu valley were collected & processed using standard microbiological techniques. All the samples were cultured on Blood agar and Macconkey agar to identify the potential pathogen. To observe Color, odor & turbidity, macroscopic observation was done. Microscopic examination was performed to observe pus cell, RBCs, epithelial cell, casts & crystals. Nitrate, protein, glucose, ketone & pH was determined by Uristrip analysis. The antibiotics sensitivity test was performed for those bacteria which were grown with significant number on culture. The colony count was performed & organisms were identified by biochemical tests.

From the study asymptomatic bacteriuria(UTI) was observed in 4 cases (7.4%)with female predominant out of total 54 samples. Among the isolates, Escherichia coli 2 (50%) was the predominant bacterial pathogen.

Study of incidence of asymptomatic UTI in primary school going children is very important in which Escherichia coli are found as major causative agents & most of the isolates were resistant to one or more antibiotics as tested in the study. 

Key words: Incidence, Asymptomatic UTI, Children, Screening, Culture, Antibiotics, Escherichia coli