Novosphingobium naphthae sp. nov., isolated from oil-contaminated soil

Novosphingobium naphthae sp. nov., isolated from oil-contaminated soil

Phase-contarst Micrograph and Transmission electron micrograph of Strain D39T

This new species of genus Novosphingobium was isolated during the study of hydrocarbon-degrading bacteria in the oil-contaminated soil of Gunsan, North Jeolla Province, South Korea. The strain is designated as D39^T. This species is a light grey-pigmented, Gram-staining negative, aerobic, non-motile, rod shaped, non-sporulating, catalase-negative, and oxidase-positive. It can grow at 20–37°C, pH 5.5–8.5, and 0–1% (w/v) NaCl concentration. 16S rRNA gene sequence analysis showed strain D39^T belongs to the genus Novosphingobium and is closely related to “Novosphingobium ginsenosidimutans” FW-6 (97.30% sequence similarity), Novosphingobium mathurense SM117^T (97.17% sequence similarity), and Novosphingobium aquiterrae E-II-3^T (97.01% sequence similarity). A polyphasic study was done to study the characteristic properties of the strain  D39^T. The quinone analysis showed the presence of ubiquinone-10 as a sole  respiratory quinone and the polyamine pattern showed presence of spermidine as a major polyamine. The polar lipid profile in TLC chromatograms revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyl-choline, sphingoglycolipid, and phosphatidyl-N-methylethanolamine. The predominant fatty acids of strain D39^T were summed feature 8 (C_18:1ω7c and/or C_18:1ω6c), summed feature 3 (C_16:1ω7c and/or C_16:1ω6c), C_17:1ω6c and C_14:0 2-OH. The genomic DNA base componenets of this novel strain was 66.7 mol%. The DNA-DNA relatedness between strain D39^T and “N. ginsenosidimutans” KACC 16015, N. mathurense KACC 14598^T, N. aquiterrae KACC 17599^T, and Novosphingobium kunmingense DSM 25975^T were 33.7%, 29.0%, 22.3% , and 18.3%, respectively Based on morphological, physiological, chemotaxonomic, and phylogenetic analyses this strain is  clearly distinguished from its closest phylogenetic neighbors. Thus, strain D39^T represents a novel species of the genus Novosphingobium, for which the name Novosphingobium naphthae sp. nov. is proposed.

The type strain is D39^T is deposited in the following culture collection centre:

KEMB 9005-346^T

KACC 18593^T

JCM 31158^T

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain D39^T is KT884900.

The article describing the novel species Novosphingobium naphthae is published in the following journal:

IJSEM:  doi: 10.1099/ijsem.0.001164

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