Incidence of Asymptomatic UTI &
Antibiotics Susceptibility Pattern of Isolates in Primary School Going
Children of Kathmandu Valley
Dhiraj
Kr. Chaudhary , Malavika Gupta, Prabin Bhandari, Reena Maharjan, Siyaram Bhagat
ABSTRACT
Urinary tract infection
is one of the most common bacterial infections during childhood. It is caused
by microbial invasion of tissue lining the urinary tract which extends from
renal cortex to urethral meatus. Asymptomatic UTI is defined as a significant bacterial
count (usually105organism/ml) present in the urine of a person
without symptoms.
The main purpose of the
study is to find out the incidence of asymptomatic UTI in primary school going
children of different age groups & sex and to know the antibiotic
susceptibility pattern of the isolates.
The study was carried
out in Microbiology Laboratory of St. Xavier’s College, Maitighar, Kathmandu
from February 6-March 21 ,2011. A total of 54 midstream urine sample of age
2-12 years children from different school of Kathmandu valley were collected
& processed using standard microbiological techniques. All the samples were
cultured on Blood agar and Macconkey agar to identify the potential pathogen.
To observe Color, odor & turbidity, macroscopic observation was done.
Microscopic examination was performed to observe pus cell, RBCs, epithelial
cell, casts & crystals. Nitrate, protein, glucose, ketone & pH was
determined by Uristrip analysis. The antibiotics sensitivity test was performed
for those bacteria which were grown with significant number on culture. The
colony count was performed & organisms were identified by biochemical
tests.
From the study
asymptomatic bacteriuria(UTI) was observed in 4 cases (7.4%)with female
predominant out of total 54 samples. Among the isolates, Escherichia
coli 2 (50%) was the predominant bacterial pathogen.
Study of incidence of
asymptomatic UTI in primary school going children is very important in which Escherichia
coli are found as major causative agents & most of the isolates were
resistant to one or more antibiotics as tested in the study.
Key
words: Incidence, Asymptomatic UTI, Children, Screening, Culture, Antibiotics,
Escherichia coli
