Wednesday, June 15, 2016

Plant Hormones: Auxin

CHAPTER: 15(B)

Plant Hormones: Auxin


Auxin:
      Auxins were the first of the major plant hormones to be discovered.
      These are a class of organic compounds which are mainly responsible for bringing about cell elongation in shoots.
      They may be produced in plants as a result of metabolism or they may be of synthetic origin.
      The principal natural auxin is indole acetic acid (IAA).
      Auxins have a cardinal role in coordination of many growth and behavioral processes in the plant's life cycle and are essential for plant body development.
      All auxins are occurred at the meristematic apices of root, shoot, buds, leaves, cotyledons, bacteria, yeast, fungi etc.

      There are several forms of auxins: Auxin –a, auxin-b, hetero auxin and related auxin are: IAA, IBA, IPA, NAA, 2, 4-D etc.



Biosynthesis of Indole Acetic acid (IAA):
      Tryptophan is the primary precursor of IAA in plants. The IAA can be formed from tryptophan by  different pathways.


  1. Indole-3-Pyruvic acid pathway:
      The amino acid tryptophan loses the amino group by deamination or transamination to form indole-3-acetic acid which then loses CO2 to form indole -3-acetaldehyde. The oxidation of indole-3-acetaldehyde results in the formation of IAA.
  1. Tryptamine pathway:
      This is the next way to synthesis IAA. In this process the tryptophan is first of all decarboxylated to form tryptamine, which is then oxidised as well as deaminated to form indoleacetaldehyde. The oxidation of indole-3-acetaldehyde results in the formation of IAA.
  1. Indole-3-aceto-nitrile pathway:
      In this pathway, tryptophan is converted into indole-3-acetaldoxime and indole-3-acetonitrile. The enzyme nitrilase is involved in this pathway. Indole-3-acetonitrile is converted to indole-3-acetic acid.

  1. Indole-3-acetamide (IAM) pathway:
      It occurs only in some bacteria.
      The tryptophan is first converted by the enzyme trp monoxygenase to Indole-3-acetamide which is then converted to IAA by the action of the enzyme IAM hydrolase.

Physiological Role of Auxin:
      Auxin promotes nuclear activities in cell enlargement.
      IAA increases the plasticity of cell wall.
      Responsible for initiation and promotion of cell division.
      Help to prevent apical dominance.
      Promotes the cambial activity.
      Useful in tissue culture.
      Involve in gene expression
      Help in protein synthesis.

Practical Application of Auxin in Agriculture:
  1. Apical Dominance:
      The influence of terminal bud in supressing the growth of laterals growing immediately below it is termed as apical dominance. If however, the apical bud is substituted by auxin , the axillary buds are inhibited which enhance its own growth.
  1. Prevention of Abscission Layer:
      The premature drop of fruits as apple, citrus, and pear can be prevented to a great extent by spraying the tree with a dilute solution of 2, 4-D, IAA, NAA or some realetd auxin.
  1. Germination:
      Auxin (IAA, IBA, and NAA) widely used to break seed dormancy and increase germination.
  1. Root Initiation:
      In vegetative propagation (cutting roots/shoots), auxins can be applied to cutting serves to induce more rooting.
  1. Flowering:
      Auxin generally inhibit flowering but enhances early flowering in pineapple, berries and barley by spraying NAA.
  1. Parthenocarpy:
      Parthenocarpy is the phenomenon of development of seedless fruits (without pollination & germination). If a flower bud is emasculated and auxin is applied to the stigma of the flower a seedless fruit develops.

  1. Tissue Culture:
      Auxin is one of the important components in tissue culture lab. It is requires for the media formulation which helps in rooting and shooting.
  1. Weed Control:
      Auxin like 2, 4-D spray can remove the weed.
  1. Sex Expression:
      The spray of auxins increases the number of female flowers in cucurbits. It helps to prevent sterility in plants.
  1. Respiartion:
      Auxin has been found to stimulate repiration.


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